Chromatography: Unveiling the Science of Separation
Chromatography is a powerful analytical technique used extensively in laboratories to separate, identify, and quantify components in a mixture. The principle behind chromatography involves the distribution of components between two phases: a stationary phase and a mobile phase. This distribution is based on differences in the affinity of each component for these phases, leading to their separation over time. There are several types of Chromatography including gas chromatography liquid chromatography thin-layer chromatography and high-performance liquid chromatography. Each type utilizes different mechanisms and apparatus but follows the same fundamental principles. In Liquid Chromatography the mobile phase is a liquid, which flows through a column packed with the stationary phase. The mixture to be analyzed is injected into this column. As the mobile phase carries the components through the column, each component interacts differently with the stationary phase, c...